COMPARATIVE STABILITY STUDY OF THYMIDINE AND (DIDEOXY-D-ERYTHRO-HEXOPYRANOSYL)THYMINE ANALOGS MONITORED BY CAPILLARY ELECTROPHORESIS

A capillary zone electrophoretic method was developed for monitoring t he stability of hexopyranosyl analogues of thymidine. The final analyt ical conditions adopted were as follows: capillary, fused silica, 70 c m (62 cm to detector) x 75 mu m I.D.; background electrolyte, 20 mM so dium tetraborate (pH 10.5); voltage, 30 kV; detection, UV at 262 nm; a nd temperature, 15 degrees C. The performance of this analytical syste m is discussed. The stability study at three pH values allows to compa re the stability of the analogues with that of thymidine and to invest igate the influence of the configuration of the N-glycosidic bond on t he rate constant of degradation.