PREPARATIVE CAPILLARY ZONE ELECTROPHORESIS OF SYNTHETIC PEPTIDES - CONVERSION OF AN AUTOSAMPLER INTO A FRACTION COLLECTOR

Preparative capillary zone electrophoresis of three synthetic peptides was performed either manually or automatically by simple manipulation s of a commercial electropherograph that is equipped only with an auto sampler without any built-in fraction collection capability. Manual fr action collection was achieved by replacing the outlet (cathode) beake r with a microcentrifuge tube, and automatic fraction collection was a ccomplished by converting the electropherograph's autosampler into a f raction collector. The latter was easily achieved mainly by the use of an extension wire, which completed the electrical circuit and facilit ated fraction collection either at a specified time or within fixed ti me intervals.