MOLECULAR-CLONING OF THE COMMON CARP (CYPRINUS-CARPIO) RHODOPSIN CDNA

Authors
TSAI HJ SHIH SR KUO CM LI LK
Citation
Hj. Tsai et al., MOLECULAR-CLONING OF THE COMMON CARP (CYPRINUS-CARPIO) RHODOPSIN CDNA, Comparative biochemistry and physiology. B. Comparative biochemistry, 109(1), 1994, pp. 81-88
Citations number
25
Categorie Soggetti
Biology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
109
Issue
1
Year of publication
1994
Pages
81 - 88
Database
ISI
SICI code
0305-0491(1994)109:1<81:MOTCC(>2.0.ZU;2-Q
Abstract
A recombinant phage clone containing a 1584 nucleotides rhodopsin cDNA was screened from a carp retinal cDNA library. The inserted DNA consi sting of a single open reading frame of 1062 nucleotides at positions 72 and 1133 encodes a 354 amino acid polypeptide. The deduced amino ac id sequence of carp rhodopsin showed 95.7, 85.5 and 74.4% identity wit h that of goldfish, sand goby and lamprey, respectively. The sites of palmitoylation, glycosylation, disulfide bond formation and Schiff bas e formation in the putative rhodopsin are all conserved.