IDENTIFICATION AND CHARACTERIZATION OF PHOSPHOENOLPYRUVATE, FRUCTOSE PHOSPHOTRANSFERASE SYSTEMS IN 3 STREPTOMYCES SPECIES

Streptomyces lividans, S. coelicolor and S. griseofuscus were examined for the presence of the enzymes of the phosphoenolpyruvate: sugar pho sphotransferase system (PTS). All three species were shown to possess Enzyme I, Hpr and fructose-specific Enzyme II (IIfru) activities. In S . lividans and S. coelicolor, all three PTS enzymes were fructose-indu cible, but in S. griseofuscus the system was expressed constructively. These organisms apparently lack the HPr(Ser) kinase and HPr(Ser-P) ph osphatase that characterize low-GC Gram-positive bacteria.