CHROMATOGRAPHIC EVALUATION OF ALKYL-BONDED PHASES PREPARED THROUGH OLEFIN HYDROSILYLATION ON A HYDRIDE SILICA INTERMEDIATE

The chromatographic characterization of alkyl-bonded silica-based stat ionary phases for HPLC is described. A previously established method i nvolving catalytic hydrosilylation of terminal olefins on a hydride-mo dified intermediate was used to obtain octyl and octadecyl packings. G enerally speaking, the resulting phases were found to be chromatograph ically equivalent (comparable retention and selectivity) to sorbents p roduced in a conventional manner using organosilanization. The new bon ded phases are, however, significantly more stable toward hydrolysis t han conventional bonded silicas. This highly desirable feature of the new bonded phases not only reduces the need for frequent column replac ement but also provides enhanced long-term reproducibility of retentio n data. The new bonded phases exhibit good separation of polycyclic ar omatic hydrocarbons, basic solutes and polypeptides.