EFFECTS OF PH AND HYDROXYPROPYL-BETA-CYCLODEXTRIN CONCENTRATION ON PEAK RESOLUTION IN THE CAPILLARY ELECTROPHORETIC SEPARATION OF THE ENANTIOMERS OF WEAK BASES

Weak base enantiomers were separated by capillary electrophoresis usin g hydroxypropyl p-cyclodextrin-containing background electrolytes. The multiple equilibria-based electrophoretic separation selectivity mode l (DID model) of enantiomers was used to analyze the results and deter mine the model parameters. The extended peak resolution equation of ca pillary electrophoresis has been applied to calculate the peak resolut ion surfaces as a function of the pH and the hydroxypropyl beta-cyclod extrin concentration of the background electrolyte, the dimensionless electroosmotic flow coefficient, and the effective portion of the appl ied potential. Combination of the DID selectivity model and the extend ed peak resolution equation allows the rational optimization of the op erating conditions and the realization of separations previously deeme d impossible.