PREPARATIVE ISOLATION OF THE LECTIN JACALIN BY ANION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The lectin jacalin from Artocarpus integrifolia was purified to homoge neity in a single step by preparative anion-exchange high-performance liquid chromatography (HPLC). Selection of the optimum chromatographic parameters in gradient elution allowed a rapid procedure to be obtain ed for the qualitative and quantitative isolation of the most importan t alpha- and alpha'-jacalin components. A recovery of 27-33% was obtai ned from a total soluble extract using a polyacrylate-DEAE HPLC column . The identities of the two isolated polypeptides were established by N-terminal amino acid sequence analysis and from the IgA(1) binding le ctin activity.