BIOCHEMICAL, HISTOCHEMICAL, AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF DISTAL TIBIAL OSTEOCHONDROSIS IN HORSES

Citation
Jd. Lillich et al., BIOCHEMICAL, HISTOCHEMICAL, AND IMMUNOHISTOCHEMICAL CHARACTERIZATION OF DISTAL TIBIAL OSTEOCHONDROSIS IN HORSES, American journal of veterinary research, 58(1), 1997, pp. 89-98
Citations number
47
Categorie Soggetti
Veterinary Sciences
ISSN journal
00029645
Volume
58
Issue
1
Year of publication
1997
Pages
89 - 98
Database
ISI
SICI code
0002-9645(1997)58:1<89:BHAICO>2.0.ZU;2-R
Abstract
Objective-To compare the biochemical, histochemical, and immunohistoch emical profiles of articular cartilage from horses with naturally acqu ired distal tibial osteochondrosis (OC) with cartilage from a similar location in clinically normal horses. Animals-9 affected horses (group 1, 16 OC lesions) and 4 control horses (group 2, 8 normal osteochondr al specimens). Procedure-OC specimens were collected during arthroscop ic removal of the fragment, and control specimens were collected by as eptic osteotomy. Uronic acid, total protein, total glycosaminoglycan ( GAG), chondroitin sulfate (CS), and keratan sulfate (KS) contents were determined. Histomorphologic, histochemical, and immunohistochemical examinations were performed on specimens after snap freezing at -80 C and cryosectioning. Monoclonal antibodies (MAB) 3B3 and 5D4 were appli ed for location of epitopes of CS and KS, respectively. Results-OC les ions had significantly lower quantity of uronic acid, total GAG, and C S, compared with normal cartilage. OC cartilage had significantly less intense staining with toluidine blue, along with irregular cellularit y and tidemark characteristics, compared with normal cartilage. Monocl onal antibodies 3B3 and 5D4 stained OC cartilage, whereas MAB 5D4 did not stain control cartilage. Additionally, MAB 3B3 and 5D4 stained the fibrous tissue that was found firmly attached to the OC lesion locate d between the parent distal portion of the tibia and OC fragment. Conc lusion-OC cartilage lesions of the distal intermediate ridge of the ti bia in horses are biochemically, histochemically, and immunohistochemi cally distinct from normal cartilage from the same location. Results m ay reflect the inability of the chondrocyte of the developing joint to alter matrix components that would allow proper maturation and differ entiation into bone.