LIQUID-CHROMATOGRAPHIC SEPARATION OF DIAMINO ANALOGS OF 2'-DEOXYADENOSINE OR 3'-DEOXYADENOSINE FROM ADENINE ON A POLY(STYRENE-DIVINYLBENZENE) POLYMER COLUMN

A liquid chromatographic method that can separate each of a series of diamino analogues of 2'- or 3'-deoxyadenosine from their main degradat ion product, adenine, is described. A PLRP-S 1000 Angstrom (8 mu m) 25 0 mm x 4.6 mm I.D. column was used at 60 degrees C. The method was dev eloped by systematic evaluation of the influence of the mobile phase p H, the type and concentration of the organic modifier, the concentrati on of the ion-pairing agent and the buffer. The mobile phase consisted of tetrahydrofuran-0.2 M sodium octanesulphonate-0.2 M potassium phos phate buffer (pH 2.0)-water (15:25:30:30, v/v).