The human hprt chromosomal region (Xq26) was physical-mapped using pul
sed field gel electrophoresis (PFGE). This work involved: (i) the reco
very of three new genomic DNA markers (DXS1327, DXS1328, and DXS1329),
(ii) the ordering of new markers relative to 11 previously available
hprt-linked markers by deletion mapping, and (iii) the completion of h
uman T-lymphocyte PFGE Southern blots using the 14 Xq26 markers. A con
tiguous 1.5-Mb physical map of the region telomeric to hprt was determ
ined. As this map identifies clusters of in vivo unmethylated rare-cut
ter restriction sites, potential CpG islands are revealed.