SIMULTANEOUS SORPTION AND ANALYTICAL DERIVATIZATION ON A POLYSTYRENE DIVINYLBENZENE POLYMER - PREPARATION OF CHROMOPHORIC AND FLUOROPHORIC DERIVATIVES OF THE PROSTAGLANDINS

A solid phase consisting of a macroreticular styrene-divinylbenzene po lymer was used as a support for the sorption and derivatization of the prostaglandins to form chromophoric and fluorophoric esters. These hi ghly potent physiological mediators are a group of 20 carbon, oxygenat ed carboxylic acids which at neutral pH are highly water soluble. In a ddition to functioning as a support for the simultaneous isolation and derivatization, the solid phase also retained the derivatized analyte s while a substantial amount of excess reagent was selectively eluted. The final clean-up was by semi-preparative chromatography.