SIMULTANEOUS SORPTION AND ANALYTICAL DERIVATIZATION ON A POLYSTYRENE DIVINYLBENZENE POLYMER - PREPARATION OF CHROMOPHORIC AND FLUOROPHORIC DERIVATIVES OF THE PROSTAGLANDINS
Jm. Rosenfeld et Xc. Fang, SIMULTANEOUS SORPTION AND ANALYTICAL DERIVATIZATION ON A POLYSTYRENE DIVINYLBENZENE POLYMER - PREPARATION OF CHROMOPHORIC AND FLUOROPHORIC DERIVATIVES OF THE PROSTAGLANDINS, Journal of chromatography, 691(1-2), 1995, pp. 231-237
A solid phase consisting of a macroreticular styrene-divinylbenzene po
lymer was used as a support for the sorption and derivatization of the
prostaglandins to form chromophoric and fluorophoric esters. These hi
ghly potent physiological mediators are a group of 20 carbon, oxygenat
ed carboxylic acids which at neutral pH are highly water soluble. In a
ddition to functioning as a support for the simultaneous isolation and
derivatization, the solid phase also retained the derivatized analyte
s while a substantial amount of excess reagent was selectively eluted.
The final clean-up was by semi-preparative chromatography.