HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF HUMAN ERYTHROCYTEOXYSTEROLS AS DELTA(4)-3-KETONE DERIVATIVES

Following oxidation by cholesterol oxidase human erythrocyte oxysterol s were analyzed as the corresponding hydroxy-Delta(4)-3-ketones by HPL C with effluent monitoring at 235 nm. Variable amounts of epimeric cho lestd-ene-3 beta, 7-diols, 3 beta-hydroxycholest-5-en-7-one, cholest-5 -ene-3 beta,19-diol, cholest-5-ene-3 beta,20-diol, cholest-5-ene-3 bet a,25-diol and cholest-5-ene-3 beta,26-diol were detected in erythrocyt e membranes of patients with sickle cell anemia or sickle cell trait. Only 3 beta-hydroxycholest-5-en-7-one was detected in erythrocyte memb ranes of healthy donors.