HYDROLYSIS OF ELASTIN BY HUMAN-LEUKOCYTE ELASTASE AND CATHEPSIN-G - INHIBITION BY BOWMAN-BIRK-TYPE SOYBEAN INHIBITOR

Authors
TIKHONOVA TV GLADYSHEVA IP KAZANSKAYA NF LARIONOVA NI
Citation
Tv. Tikhonova et al., HYDROLYSIS OF ELASTIN BY HUMAN-LEUKOCYTE ELASTASE AND CATHEPSIN-G - INHIBITION BY BOWMAN-BIRK-TYPE SOYBEAN INHIBITOR, Biochemistry, 59(11), 1994, pp. 1295-1299
Citations number
20
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
59
Issue
11
Year of publication
1994
Pages
1295 - 1299
Database
ISI
SICI code
0006-2979(1994)59:11<1295:HOEBHE>2.0.ZU;2-O
Abstract
Human leukocyte cathepsin G stimulates the hydrolysis of bovine neck l igament elastin by leukocyte elastase. The stimulation factor (1.0-2.0 ) depends on the enzyme concentration ratio and ionic strength. Soybea n Bowman-Birk-type inhibitor effectively inhibits the hydrolysis of el astin by elastase, cathepsin G, or a mixture of both enzymes. The effi ciency of inhibition was virtually independent of ionic strength, the ratio between the enzyme concentrations, their preadsorption on elasti n, or the stage of hydrolysis of elastin.