DEVELOPMENT OF BONE-MARROW IMMUNOSCINTIGRAPHY USING A TC-99M LABELED ANTI-NCA-95 MONOCLONAL-ANTIBODY

Authors
CHOI CW CHUNG JK LEE DS LEE MC CHUNG HK KIM BK KOH CS
Citation
Cw. Choi et al., DEVELOPMENT OF BONE-MARROW IMMUNOSCINTIGRAPHY USING A TC-99M LABELED ANTI-NCA-95 MONOCLONAL-ANTIBODY, Nuclear medicine and biology, 22(1), 1995, pp. 117-123
Citations number
22
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
Journal title
Nuclear medicine and biology
ISSN journal
09698051 → ACNP
Volume
22
Issue
1
Year of publication
1995
Pages
117 - 123
Database
ISI
SICI code
0969-8051(1995)22:1<117:DOBIUA>2.0.ZU;2-G
Abstract
We evaluated the monoclonal antibody CEA-79.4 against carcinoembryonic antigen as an immunoscintigraphic agent for assessing the state of th e bone marrow. Western blotting of human granulocyte extracts with the antibody could confirm that the binding was with the epitope of NCA-9 5. Immunocytochemical staining of bone marrow aspirates revealed speci fic uptake of this antibody by granulopoietic cells. The affinity cons tant was 2-9 x 10(9) L/mol. Immunoscintigraphy using Tc-99m-labeIed CE A-79.4 in a normal Volunteer revealed high uptake in the bone marrow a s compared to other organs.