N. Utoguchi et al., ISOLATION AND PROPERTIES OF TUMOR-DERIVED ENDOTHELIAL-CELLS FROM RAT KMT-17 FIBROSARCOMA, Japanese journal of cancer research, 86(2), 1995, pp. 193-201
Rat KMT-17 fibrosarcoma-derived endothelial cells were isolated by Per
coll gradient centrifugation with an attaching-speed separation techni
que, and their properties in culture were examined. The primary cultur
ed tumor-derived endothelial cells (TEC) showed angiotensin-converting
enzyme activity, positivity for Factor VIII-related antigen staining,
and typical capillary-like formation on Matrigel. The primary culture
d TEC monolayer showed greater permeability than normal tissue-derived
endothelial cell (aorta, vena cava and epididymal fat capillary) mono
layers on FITC-dextran diffusion (molecular weight 70,000). Leukocyte
adhesion to TEC was reduced compared to that to fat-derived capillary
endothelial cells. These characteristics resembled those of tumor vasc
ular endothelium, and were observed both in the primary and first-pass
age cell cultures, but not in the fourth-passage cell cultures. Our fi
ndings indicate that primary or subcultured TEC are applicable for stu
dies of the physiological characteristics of tumor endothelial cells.