CHROMOSOME-DAMAGE INDUCED BY COMBINED TREATMENTS WITH RESTRICTION ENDONUCLEASES INTRODUCED INTO CHO CELLS BY SINGLE OR DOUBLE ELECTROPORATION

Citation
T. Ortiz et al., CHROMOSOME-DAMAGE INDUCED BY COMBINED TREATMENTS WITH RESTRICTION ENDONUCLEASES INTRODUCED INTO CHO CELLS BY SINGLE OR DOUBLE ELECTROPORATION, Mutation research, 327(1-2), 1995, pp. 161-169
Citations number
23
Categorie Soggetti
Genetics & Heredity",Biology,"Biothechnology & Applied Migrobiology
Journal title
ISSN journal
00275107
Volume
327
Issue
1-2
Year of publication
1995
Pages
161 - 169
Database
ISI
SICI code
0027-5107(1995)327:1-2<161:CIBCTW>2.0.ZU;2-3
Abstract
The possible recombination between non-homologous termini produced by restriction enzymes (REs) introduced in CHO cells by electroporation w as studied. For this purpose, different combinations of REs that produ ced blunt or 5' overhanging DNA double-strand breaks were electroporat ed into cells either at the same time or separately by double electrop oration experiments. Prior to double electroporation, it was confirmed that, once the cells have been electroporated, they resist a second e lectroporation, as assessed by cell viability analysis. Besides, the e fficient and homogeneous introduction of labelled, non-permeable molec ules was assessed by fluorescence microscopy. Our results showed inter action for most of the conditions, mainly when the REs were introduced separately. Differences found in the degree of interaction between th e combinations studied are discussed.