T. Ortiz et al., CHROMOSOME-DAMAGE INDUCED BY COMBINED TREATMENTS WITH RESTRICTION ENDONUCLEASES INTRODUCED INTO CHO CELLS BY SINGLE OR DOUBLE ELECTROPORATION, Mutation research, 327(1-2), 1995, pp. 161-169
The possible recombination between non-homologous termini produced by
restriction enzymes (REs) introduced in CHO cells by electroporation w
as studied. For this purpose, different combinations of REs that produ
ced blunt or 5' overhanging DNA double-strand breaks were electroporat
ed into cells either at the same time or separately by double electrop
oration experiments. Prior to double electroporation, it was confirmed
that, once the cells have been electroporated, they resist a second e
lectroporation, as assessed by cell viability analysis. Besides, the e
fficient and homogeneous introduction of labelled, non-permeable molec
ules was assessed by fluorescence microscopy. Our results showed inter
action for most of the conditions, mainly when the REs were introduced
separately. Differences found in the degree of interaction between th
e combinations studied are discussed.