THE OUTER-MEMBRANE PROTEIN E24 OF SPINACH CHLOROPLAST ENVELOPE - CLONING OF A CDNA AND TOPOLOGICAL INSERTION OF THE PROTEIN IN THE MEMBRANE

Authors
TEYSSIER E BLOCK MA GARIN J JOYARD J DOUCE R
Citation
E. Teyssier et al., THE OUTER-MEMBRANE PROTEIN E24 OF SPINACH CHLOROPLAST ENVELOPE - CLONING OF A CDNA AND TOPOLOGICAL INSERTION OF THE PROTEIN IN THE MEMBRANE, Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 318(1), 1995, pp. 17-25
Citations number
23
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie → ACNP
ISSN journal
07644469
Volume
318
Issue
1
Year of publication
1995
Pages
17 - 25
Database
ISI
SICI code
0764-4469(1995)318:1<17:TOPEOS>2.0.ZU;2-B
Abstract
A cDNA coding for the outer membrane protein E24 of spinach chloroplas t envelope has been obtained by screening an expression library of spi nach cDNA with polyclonal antibodies. Analysis of the protein sequence and comparison with the thermolysin susceptibility of E24 in the chlo roplast in situ suggest that E24 is a transmembrane protein and show t hat its NH2 terminal end is located on the cytosolic side of chloropla sts.