APPLICATION OF MINERAL SUPPORT ON CEPHAMYCIN-C PRODUCTION IN CULTURE USING SOYBEAN OIL AS THE SOLE CARBON SOURCE

A mineral support was used for cephamycin C production in a culture us ing soybean oil as the sole carbon source. When the support was added into an oil-water system, the soybean oil was emulsified as fine oil d roplets, which was observed by a photomicroscope. Mycelia were also tw ined around the support, which was observed by a scanning electron mic roscope. That caused the formation of an oil-mycelia complex on or aro und the support, which provided a larger specific surface area of oil. When 15 g/L of the support was used in a batch culture of Streptomyce s sp. P6621 with 50 g/L of soybean oil, the maximum cephamycin C conce ntration was 2.8 g/L, which was 2.2 times higher than that without the support. This indicates that the mineral support is useful for the cu lture system using vegetable oil as a carbon source. (C) 1997 John Wil ey & Sons, Inc.