PARTIAL-PURIFICATION OF PLASMA PHENOLOXIDASE OF THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI L (DIPTERA, CULICIDAE)

Authors
BURKS CS FUCHS MS
Citation
Cs. Burks et Ms. Fuchs, PARTIAL-PURIFICATION OF PLASMA PHENOLOXIDASE OF THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI L (DIPTERA, CULICIDAE), Comparative biochemistry and physiology. B. Comparative biochemistry, 110(3), 1995, pp. 641-647
Citations number
21
Categorie Soggetti
Biology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
110
Issue
3
Year of publication
1995
Pages
641 - 647
Database
ISI
SICI code
0305-0491(1995)110:3<641:POPPOT>2.0.ZU;2-R
Abstract
A nitrocellulose-based assay was de,eloped using a dot-blot apparatus to detect phenoloxidase activity in column fractions. Using this assay , plasma phenoloxidase was partially purified from Aedes aegypti larva e using hydrophobic interaction chromatography, gel filtration, and io n-exchange chromatography. The molecular weight (M(r)) native enzyme w as 130,000, and it contained subunits of 76,000, 62,000, and 58,000. T wo phenoloxidase peaks were observed by ion exchange chromatography, a nd these Fractions had distinct polypeptide profiles as detected by SD S-PAGE.