Cs. Burks et Ms. Fuchs, PARTIAL-PURIFICATION OF PLASMA PHENOLOXIDASE OF THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI L (DIPTERA, CULICIDAE), Comparative biochemistry and physiology. B. Comparative biochemistry, 110(3), 1995, pp. 641-647
A nitrocellulose-based assay was de,eloped using a dot-blot apparatus
to detect phenoloxidase activity in column fractions. Using this assay
, plasma phenoloxidase was partially purified from Aedes aegypti larva
e using hydrophobic interaction chromatography, gel filtration, and io
n-exchange chromatography. The molecular weight (M(r)) native enzyme w
as 130,000, and it contained subunits of 76,000, 62,000, and 58,000. T
wo phenoloxidase peaks were observed by ion exchange chromatography, a
nd these Fractions had distinct polypeptide profiles as detected by SD
S-PAGE.