COMPARATIVE BIOCHEMICAL-STUDY OF COATED VESICLES PURIFIED FROM COCK BRAINS

Authors
YORA T SAKAGISHI Y NAKAMURA H
Citation
T. Yora et al., COMPARATIVE BIOCHEMICAL-STUDY OF COATED VESICLES PURIFIED FROM COCK BRAINS, Comparative biochemistry and physiology. B. Comparative biochemistry, 110(2), 1995, pp. 331-337
Citations number
29
Categorie Soggetti
Biology
Journal title
Comparative biochemistry and physiology. B. Comparative biochemistry → ACNP
ISSN journal
03050491
Volume
110
Issue
2
Year of publication
1995
Pages
331 - 337
Database
ISI
SICI code
0305-0491(1995)110:2<331:CBOCVP>2.0.ZU;2-K
Abstract
Cock brain coated vesicles (CBCVs) were purified and compared with por cine brain coated vesicles (PBCVs) from several biochemical aspects. C lathrin heavy and light chains of CBCVs were immunologically similar t o those of PBCVs. Coat proteins (CPs) of CBCVs behaved in almost the s ame manner as those of PBCVs for limited proteolysis. Dissociation of CPs from CBCVs by treatment with 10 mM Tris-Cl, pH 8.5, or 2 M urea re sembled that of CPs from PBCVs. pH dependency of dissociation of CPs f rom CBCVs was slightly different from those of PBCVs.