A. Ekmekci et al., IN-VITRO EFFECTS OF PROSTAGLANDIN-E1 AND INDOMETHACIN ON MITOMYCIN C-INDUCED SISTER-CHROMATID EXCHANGES IN MITOGEN-STIMULATED HUMAN-LYMPHOCYTES, Mutation research, 328(1), 1995, pp. 49-53
In this study, the individual and combined effects of prostaglandin E1
(PGE1) and Indomethacin on mitomycin C (MMC)-induced SCEs in human ly
mphocytes was investigated in vitro. All MMC-treated cultures showed a
great increase of SCEs (approximately two-fold), indicating its abili
ty to induce mutations. SCE data showed that MMC-induced SCEs were red
uced significantly in the presence of PGE1 in pooled analysis of six e
xperiments (60.55% reduction of SCEs at 10(-6) M, 34.13% reduction of
SCEs at 10(-9) M). In contrast the presence of indomethacin in the med
ium during MMC treatment of cells failed to show a significant reducti
on of SCEs in pooled analysis (21.17%). However, individual analyses r
evealed only two of six donors with a significant SCE response. Thus,
the findings suggest that PGE1 can modify the DNA damaging effect of c
arcinogens and thereby may prevent the initiation of the carcinogenic
process.