IN-VITRO EFFECTS OF PROSTAGLANDIN-E1 AND INDOMETHACIN ON MITOMYCIN C-INDUCED SISTER-CHROMATID EXCHANGES IN MITOGEN-STIMULATED HUMAN-LYMPHOCYTES

In this study, the individual and combined effects of prostaglandin E1 (PGE1) and Indomethacin on mitomycin C (MMC)-induced SCEs in human ly mphocytes was investigated in vitro. All MMC-treated cultures showed a great increase of SCEs (approximately two-fold), indicating its abili ty to induce mutations. SCE data showed that MMC-induced SCEs were red uced significantly in the presence of PGE1 in pooled analysis of six e xperiments (60.55% reduction of SCEs at 10(-6) M, 34.13% reduction of SCEs at 10(-9) M). In contrast the presence of indomethacin in the med ium during MMC treatment of cells failed to show a significant reducti on of SCEs in pooled analysis (21.17%). However, individual analyses r evealed only two of six donors with a significant SCE response. Thus, the findings suggest that PGE1 can modify the DNA damaging effect of c arcinogens and thereby may prevent the initiation of the carcinogenic process.