Uh. Ehling et A. Neuhauserklaus, INDUCTION OF SPECIFIC-LOCUS AND DOMINANT LETHAL MUTATIONS IN MALE-MICE BY N-PROPYL AND ISOPROPYL METHANESULFONATE, Mutation research, 328(1), 1995, pp. 73-82
n-Propyl methanesulfonate (nPMS) and isopropyl methanesulfonate (iPMS)
induce dominant lethal and specific-locus mutations in male mice. The
responses of the various spermatogenic stages to the induction of mut
ations differ markedly for nPMS and iPMS. Independent of the effective
dose range the induction of dominant lethal mutations by nPMS is limi
ted to spermatozoa and spermatids. In contrast, the induction of domin
ant lethal mutations by iPMS is dose dependent: a dose of 20 mg iPMS/k
g body weight (bw) is active only in spermatocytes, while a dose of 10
0 mg/kg bw induces dominant lethal mutations in all postspermatogonial
germ cell stages. One other striking difference in the biological eff
ectiveness of both compounds is that iPMS induces a sterile phase in s
tem-cell spermatogonia, whereas nPMS treated males even at the highest
dose are fully fertile.