IMPROVEMENT OF BLOOD AND FLY GUT PROCESSING FOR PCR DIAGNOSIS OF TRYPANOSOMOSIS

Authors
PENCHENIER L DUMAS V GREBAUT P REIFENBERG JM CUNY G
Citation
L. Penchenier et al., IMPROVEMENT OF BLOOD AND FLY GUT PROCESSING FOR PCR DIAGNOSIS OF TRYPANOSOMOSIS, Parasite, 3(4), 1996, pp. 387-389
Citations number
9
Categorie Soggetti
Parasitiology
Journal title
Parasite → ACNP
ISSN journal
1252607X
Volume
3
Issue
4
Year of publication
1996
Pages
387 - 389
Database
ISI
SICI code
1252-607X(1996)3:4<387:IOBAFG>2.0.ZU;2-N
Abstract
We have adapted a simple and efficient technique to detect trypanosome s in human blood, without DNA purification, and increased the sensitiv ity threshold to 1 parasite in 1 ml. We have then applied it for detec tion of parasites in midguts of tsetse flies, negative by microscopy. This technique has been developed for field conditions and could great ly facilitate epidemiological studies.