RETENTION PROPERTIES OF TRIACYLGLYCEROLS ON SILVER ION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The retention properties of series of synthetic disaturated-monounsatu rated (1 to 6 double bonds) triacylglycerols and the common seed oil t riacylglycerols on a silver ion high-performance liquid chromatography column have been studied in quantitative terms. Retention factors wer e found to increase stepwise, with a saturated dimonoenoic species bei ng held ten times and trilinolenin ten thousand times more strongly th an a disaturated monoenoic species, for example. Formation of chelate- type of pi-complexes between silver ions and double bonds, with partic ipation of the carbonyl oxygen in the complexation, have been assumed in order to explain the high separation power and specificity of the s ilver ion column.