COMPARATIVE-STUDY ON SALMONELLA MUTAGENICITY AND ON CYTOGENETIC AND ANTINEOPLASTIC EFFECTS INDUCED BY CYCLOPHOSPHAMIDE AND 3-AMINOBENZAMIDEIN CELLS OF 3 TRANSPLANTABLE TUMORS IN-VIVO

Synergistically enhanced sister chromatid exchange (SCE) frequency by cyclophosphamide (CP) was observed when L1210 lymphoid tumor cells wer e exposed in vivo to a non-toxic concentration of 3-aminobenzamide (3- AB). Additive effects in SCE induction in vivo were observed when eith er Ehrlich ascites tumor (EAT) cells or P388 lymphocytic leukemia cell s treated with CP were exposed to 3-AB in vivo. 3-AB enhanced the surv ival time of L1210 tumor bearing BDF1 mice treated with CP. However, t he combined CP plus 3-AB treatment did not increase the survival of ei ther EAT BALB/c- or P388 BDF1-tumor bearing mice compared with the eff ect on survival by CP alone. Therefore the in vivo differential antitu mor effect, by CP in conjunction with 3-AB, appears to correlate well with the in vivo differential effect on cytogenetic damage caused by t he combined CP plus 3-AB treatment. In the Salmonella typhimurium/mamm alian microsome test CP appears to have a dose dependent ability to in duce base-pair substitutions in strains TA 100 and TA 1535 and framesh ift mutations in strains TA 98 and TA 1537. Both types of mutation wer e synergistically increased in the presence of 3-AB.