Ks. Hsu et Sy. Linshiau, ANTAGONISM BY PARAQUAT OF THE RELAXING EFFECTS OF ACETYLCHOLINE AND A23187 IN RAT THORACIC AORTA, European journal of pharmacology. Environmental toxicology and pharmacology section, 292(3-4), 1995, pp. 315-320
Paraquat, a widely used herbicide, has been reported to be capable of
producing superoxide. In the present paper, therefore, the possibility
of paraquat inhibiting endothelium-dependent relaxation, mediated by
the production of nitric oxide, was tested. The relaxing effects of ac
etylcholine and A23187, but not that of sodium nitroprusside, in the r
at thoracic aorta were found to be inhibited by paraquat in a concentr
ation-dependent manner. In contrast, paraquat was totally inactive wit
h regard to the aortic contractions induced by either norepinephrine o
r prostaglandin F-2 alpha. The inhibitory action of paraquat could be
antagonized by superoxide dismutase but not by catalase and indomethac
in. All of these findings indicate that superoxide anions produced by
paraquat in the endothelium contribute to a decrease in the relaxation
response to acetylcholine and A23187 by interfering with endothelium-
derived nitric oxide.