APPLICABILITY OF COUPLED-COLUMN LIQUID-CHROMATOGRAPHY TO THE ANALYSISOF BETA-AGONISTS IN URINE BY DIRECT SAMPLE INJECTION .1. DEVELOPMENT OF A SINGLE-RESIDUE REVERSED-PHASE LIQUID-CHROMATOGRAPHY UV METHOD FORCLENBUTEROL AND SELECTION OF CHROMATOGRAPHIC CONDITIONS SUITABLE FOR MULTI-RESIDUE ANALYSIS
A. Polettini et al., APPLICABILITY OF COUPLED-COLUMN LIQUID-CHROMATOGRAPHY TO THE ANALYSISOF BETA-AGONISTS IN URINE BY DIRECT SAMPLE INJECTION .1. DEVELOPMENT OF A SINGLE-RESIDUE REVERSED-PHASE LIQUID-CHROMATOGRAPHY UV METHOD FORCLENBUTEROL AND SELECTION OF CHROMATOGRAPHIC CONDITIONS SUITABLE FOR MULTI-RESIDUE ANALYSIS, Journal of chromatography, 695(1), 1995, pp. 19-31
Optimisation procedures originally applied to coupled-column RPLC-UV f
or the residue analysis of polar pesticides were evaluated for the ana
lysis of beta-agonists in human and bovine urine using direct sample i
njection. Two approaches have been studied: (i) a multi-residue method
(MRM) for the clean-up and separation of eight different beta-agonist
s (isoprenaline, cimaterol, terbutaline, salbutamol, fenoterol, ractop
amine, clenbuterol and mabuterol) and (ii) a single-residue method (SR
M) focussed at the detection of clenbuterol residues in samples of uri
ne. Both approaches provided efficient procedures to process urine sam
ples automatically with coupled-column LC. Particular attention was pa
id to selecting analytical conditions suitable for thermospray MS dete
ction, which is to be investigated in the near future. Though UV detec
tion cannot offer enough selectivity for the simultaneous screening of
a group of p-agonists, coupled-column RPLC-UV proved to be very power
ful in SRM, allowing the detection of clenbuterol at the mu g/l level
in filtered (0.35 0.45 mu m) human and bovine urine after direct sampl
e injection.