MIXED ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF URIDINE 5'-DIPHOSPHO-ALPHA-D-GLUCURONIC ACID AND ITS HYDROLYSIS PRODUCTS

Although conventional ion-exchange HPLC and ion-pair reversed-phase HP LC using tetrabutylammonium hydroxide allowed ready determination of u ridine 5'-diphospho-alpha-D-glucose and its hydrolysis products, neith er method was suitable for the determination of uridine 5'-diphospho-a lpha-D-glucuronic acid (alpha-UDPGA). However, mixed ion-pair reversed -phase HPLC, using a combination of tetrabutylammonium hydroxide and t etraethylammonium hydroxide (1:1), has been found to offer a convenien t and effective means of simultaneously determining alpha-UDPGA and it s hydrolysis products, UDP, UMP and cUMP.