PREPARATIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF FLUORODEOXY SUGARS

Normal- and reversed-phase preparative chromatography methods were dev eloped to isolate gram quantities of analytically pure -deoxy-2-fluoro -beta-D-arabinofuranosyl)-9H-purine (arafluoro-2-CdA; RWJ 29727) and i ts alpha-anomer (RWJ 48667). The complex reaction mixture (ca. 171 g), from a Parr Bomb synthesis, was prepurified by normal-phase chromatog raphy to yield ca. 40 g. Twelve reversed-phase preparative isolations were run on a custom-packed YMC column to yield ca. 12 g of arafluoro- 2-CdA (99.7%) and ca. 3 g of the cr-anomer (99.2%).