INVESTIGATION OF EXPERIMENTAL ERRORS IN THE QUANTITATIVE-ANALYSIS OF GLUCOSE IN HUMAN BLOOD-PLASMA BY ATR-IR SPECTROSCOPY

The determination of glucose in physiological fluids such as whole blo od or blood plasma can be carried out by using mid-infrared spectromet ry in combination with multivariate calibration. The preferred techniq ue for such measurements is the attenuated total reflection technique (ATR). A variance analysis recently published showed that the spectros copic conditions contribute significantly to the total prediction vari ance of the calibration models. Various effects such as temperature va riation, cell filling, protein adsorption have been investigated for t heir influence on the spectra.