NEAR-INFRARED LASER-INDUCED FLUORESCENCE DETECTION IN COLUMN LIQUID-CHROMATOGRAPHY - A COMPARISON OF VARIOUS LASERS AND DETECTION SYSTEMS .1. CONTINUOUS-WAVE LASERS
Ajg. Mank et al., NEAR-INFRARED LASER-INDUCED FLUORESCENCE DETECTION IN COLUMN LIQUID-CHROMATOGRAPHY - A COMPARISON OF VARIOUS LASERS AND DETECTION SYSTEMS .1. CONTINUOUS-WAVE LASERS, Journal of chromatography, 695(2), 1995, pp. 165-174
Several lasers, i.e. HeNe lasers, diode lasers and an argon-ion-dye la
ser combination, are compared as excitation sources for near-infrared
laser-induced fluorescence detection in column liquid chromatography.
Using a model gradient liquid chromatography system, detection limits
for disulphonated aluminum phthalocyanine (AlPcS2) were compared after
optimization of the signal processing scheme for each laser. The best
detection limit (1.5 . 10(-13) M) was obtained for the argon-ion-dye
laser combination, applying ratioing of emission and excitation light
to reduce flicker noise. Utilizing diode laser excitation there is;no
need far ratioing so that emission collection can be improved and a de
tection limit similar to that obtained with the argon-ion laser can be
achieved. As this device is very cheap, the diode laser is the best c
hoice for most applications. If tunability over a large wavelength reg
ion is essential, the argon-ion-dye laser combination is a good but ex
pensive alternative.