Ja. Gerstner et al., RAPID ION-EXCHANGE DISPLACEMENT CHROMATOGRAPHY OF PROTEINS ON PERFUSIVE CHROMATOGRAPHIC SUPPORTS, Journal of chromatography, 695(2), 1995, pp. 195-204
Displacement chromatography is an extremely powerful separation techni
que. However, it has not been widely accepted as a preparative techniq
ue due to the creeping flow-rates at which displacement separations ha
ve been demonstrated. In this work, the enhanced mass transport of per
fusive chromatographic particles enabled displacement separations to b
e dramatically scaled-up with respect to flow-rate. At a flaw-rate of
4 ml/min (1440 cm/h) on an analytical-scale column, crude beta-lactogl
obulin was displaced in only 90 s to yield ca. 18 mg each of pure beta
-lactoglobulin A and B. This represents an order of magnitude decrease
in the time required for displacement separations. Thus, the operatio
n of perfusive columns in the displacement mode offers an ultra-high t
hroughput separation technique with outstanding resolving power even u
nder heavily overloaded conditions.