ONLINE KINETIC MONITORING FOR BIOCHEMICAL REACTIONS USING MULTIPOINT DETECTION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

The potential utility of high-performance capillary electrophoresis fo r on-line kinetic monitoring of biochemical reactions is demonstrated using a multi-point detection method. The adenosine deaminase-catalyze d deamination of adenosine to inosine is studied. The deamination spee d increases with the increase in enzyme concentration in the run buffe r. Degradation of heme protein, myoglobin, has been investigated by th is method. Under certain electrophoretic conditions, the degradation f ollows first-order kinetics. The degradation rate constant increases w ith an increase in temperature and the electric field applied to the c apillary does not appreciably effect the degradation rate.