E. Strobel et al., ISOANTIBODIES IN INTRAVENOUS IMMUNOGLOBUL INS - DETECTION AND SIDE-EFFECTS, Infusionstherapie und Transfusionsmedizin, 22(1), 1995, pp. 31-35
Background: Intravenous immunoglobulins (IvIg) contain not only the de
clared antibodies against pathogenic microorganisms, but also all the
other antibodies of the blood donors, e. g. against erythrocytic antig
ens. Materials and Methods: We tested 14 IvIg from 7 manufacturers (a
total of 40 charges) for isoantibodies and irregular antibodies. To im
prove the reading of our tests we used the gel centrifugation method (
ID-Microtyping-System(R), Fa. Diamed, Bensheim, Germany). Results: The
highest isoantibody titers were (in 8 charges) 1:32 or 1:64 in the Li
ss-Coombs test. Irregular antibodies were found in 5 IvIg (maximal tit
er 1:8). Conclusions: Isoantibodies in the IvIg can influence blood gr
oup serologic tests. With an example of a newborn who had received IvI
g we point to the potential danger of misinterpretation of a positive
direct antiglobulin test after administration of IvIg. Therefore we re
commend to carry out the direct antiglobulin test before administratio
n of IvIg and to examine all eluates after a positive direct Coombs te
st not only with O RBCs but also with A or B RBCs of the ABO blood gro
up of the patient.