SITE-DIRECTED CLEAVAGE OF SINGLE INTERNUCLEOTIDE BONDS IN RIBOSOMAL-RNA

Authors
BOGDANOVA SL DEGTYAREV AI BARANOV PV DOKUDOVSKAYA SS LAVRIK IN DONTSOVA OA ORETSKAYA TS KRYNETSKAYA NF SHABAROVA ZA BOGDANOV AA
Citation
Sl. Bogdanova et al., SITE-DIRECTED CLEAVAGE OF SINGLE INTERNUCLEOTIDE BONDS IN RIBOSOMAL-RNA, Biochemistry, 60(2), 1995, pp. 217-224
Citations number
18
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
60
Issue
2
Year of publication
1995
Pages
217 - 224
Database
ISI
SICI code
0006-2979(1995)60:2<217:SCOSIB>2.0.ZU;2-S
Abstract
The cleavage of 16S ribosomal RNA (rRNA) from E. coli with ''hammerhea d'' ribozymes and with RNase H in the presence of ''chimeric'' (2'-deo xy-F-thymidine containing) oligonucleotides has been studied. The cond itions for the cleavage of a desired single internucleotide bond in th ese large molecules with very complicated secondary and tertiary struc tures have been found.