IS292 - A NOVEL INSERTION ELEMENT FROM AGROBACTERIUM

A new insertion sequence, IS292, located in the 6b gene of a nopaline- type Agrobacterium strain (X88-292) isolated from poplar was identifie d and sequenced. IS292 is 2494 bp long, has 21 bp inverted terminal re peats with two mismatches, and generates 10 bp direct repeats upon int egration. No sequence similarity was found between IS292 and other ins ertion elements associated with Agrobacterium, but it shows strong sim ilarity with ISRl1 from Rhizobium leguminosarum by. viciae. The occurr ence of IS292-like sequences in various Agrobacterium isolates, especi ally different Agrobacterium strains isolated from the same biotope, w as demonstrated by DNA hybridization.