THEORETICAL INTERPRETATION OF THE RETENTION OF SYSTEM PEAKS IN PARTITION CHROMATOGRAPHY WITH A MOBILE-PHASE CONTAINING ELECTROLYTES

Theoretical equations were developed to describe the retention depende nce of system peaks generated in partition chromatography with a mobil e phase containing electrolytes on the composition of the mobile phase electrolytes, on the basis of the model regarding the partitioning of ionic solutes and Knox and Kaliszan's general equations for the reten tion of system peaks. The predictions from the equations agreed well w ith experimental results obtained on hydrophilic polymer packings for both water and acetone-water mobile phase systems containing two or th ree background electrolytes. This means that the retention of system p eaks can be predicted even for three or more mobile phase component sy stems, once the dependence of the distribution of each mobile phase co mponent between the mobile and stationary phases on the composition of the mobile phase has been elucidated.