QUANTITATIVE-DETERMINATION OF ORGANIC-SOLVENTS BY CAPILLARY ELECTROPHORESIS USING INDIRECT UV DETECTION

A sodium dodecyl sulphate (SDS)-based micellar electrokinetic capillar y chromatography method is reported for the quantitative determination of a range of simple organic solvents employing indirect UV absorbanc e detection. Veronal buffer is used to provide both pH buffering and t he background UV signal for indirect detection. The concentrations of both SDS and veronal buffer were optimized using an experimental desig n approach. The method was shown to give acceptable performance in ter ms of various performance parameters including selectivity, linearity, precision and detection limits. The method was succesfully applied to the determination of the ethanol content in pharmaceutical formulatio ns and alcoholic beverages. Good correlation was obtained between the CE results and the label claims. The method has several features inclu ding simplicity, short analysis time and robustness.