Hairless (SKH-1) mice were mated with Beige (C57B/bb) mice to produce
a hairless mouse deficient in neutrophil elastase (hhbb). These mice w
ere exposed to 0.091 UVB irradiation for 5 months to see if neutrophil
elastase was an important factor in elastin remodeling and developmen
t of solar elastoses. Analysis of peritoneal neutrophils confirmed mat
the hhbb mouse was deficient in elastase, retaining only 10% as much
activity as the normal littermates (hhHb). Skin MPO activity was equal
ly elevated in all the mice receiving UVB suggesting an equal influx o
f inflammatory cells. The absolute breaking strength of the skin in bo
th the hhBb and hhbb mice was not altered by UVB treatment over the 5
month exposure period. Elastin quantitated biochemically as desmosine,
or visualized histologically, was increased following WE exposure in
the normal mice. In the elastase deficient mice, however, the elastin
fibers appeared to be unaffected by exposure to UVB irradiation at thi
s level. The results suggest that neutrophil elastase is an important
mediator in the development of solar elastosis resulting from continue
d exposure to UVB irradiation.