PERFORMANCE OF REVERSED-PHASE PARALLEL-CURRENT OPEN-TUBULAR LIQUID-CHROMATOGRAPHY COLUMNS AND COMPARISON WITH THEORY

The capillary column performance in reversed-phase parallel-current op en-tubular liquid chromatography (RP-PC-OTLC) was studied with the use of cyclohexanol-water, The reduced retention and the height equivalen t to a theoretical plate were studied as a function of the liquid velo city and the content of cyclohexanol in the capillary at constant colu mn temperature. The effect of microemulsion formation on band broadeni ng and retention is discussed. The plate heights obtained were compare d with the Golay equation.