APPLICATION OF A COMPARATIVE-EVALUATION OF SEVERAL REVERSED-PHASE COLUMNS TO THE AUTOMATED-ANALYSIS OF CANDIDATE PHARMACEUTICALS

This report discusses the evaluation of several modern reversed-phase chromatographic packing materials, and describes the application of a selected column in a method used to screen the purities of pharmaceuti cal candidates and intermediates. Columns are first evaluated with an acetonitrile gradient using a test mixture containing acids, bases and compounds of widely differing polarities. Selected phases are further assessed by analysing many typical samples. Finally, the use of the c hosen column and method are described and discussed.