PREPARATIVE-SCALE SUPERCRITICAL-FLUID CHROMATOGRAPHY

An apparatus for the study of preparative supercritical fluid chromato graphy (SFC) has been built and used to study the separation of a mode l system [a 1:1 (w/w) mixture of fluorene and phenanthrene] and a more real system (a mixture of milbemycins containing also other extracted material). The principles of preparative SFC are given and applied to these studies, and some conclusions about the optimisation of prepara tive SFC drawn.