Ms. Paraiso et al., PARATHYROID-HORMONE INHIBITS PLASMA-MEMBRANE PI-TRANSPORT WITHOUT CHANGING ENDOCYTIC ACTIVITY IN OPOSSUM KIDNEY-CELLS, Biochimica et biophysica acta. Molecular cell research, 1266(2), 1995, pp. 143-147
Parathyroid hormone (PTH) inhibits Na+-dependent Pi uptake in renal ep
ithelial cells from opossum kidney (OK). This requires an intact endoc
ytic pathway, suggesting that one action of PTH may be to promote endo
cytic removal of Na+/Pi cotransporters from the cell membrane. The pre
sent study tested if PTH, at a dose that inhibited membrane Pi transpo
rt, also produced an increase in endocytic activity. Pi transport was
measured in isolated plasma membrane vesicles. Endocytosis was measure
d by allowing cells to take up horseradish peroxidase (HRP) followed b
y assay of triton-sensitive (latent) HRP activity in subcellular fract
ions isolated by density gradient centrifugation. Incubation of OK cel
ls with 10(-7) M PTH for 3 h decreased Na+/Pi cotransport by membrane
vesicles to 328 +/- 54 pmol/mg/min compared to 448 +/- 67 pmol/mg/min
(mean +/- S.E., P < 0.03) in controls. Latent HRP content of endosomal
fractions was dependent on the time and temperature used to load cell
s with HRP and on the concentration of HRP. However, incubation of OK
cells with 10(-7) M PTH for either 1 or 3 h produced no change in late
nt HRP activity. Thus the action of PTH on the Na+/Pi cotransporter in
the plasma membrane of OK cells does not require a change in the rate
of endocytosis.