LECTIN AFFINITY IMMUNOELECTROPHORESIS OF SERUM GLYCOPROTEINS

The basic methodology of two major affinity immunoelectrophoresis tech niques, i.e., crossed affinity immunoelectrophoresis and lectin affini ty electrophoresis with immunoblotting, is described. This review cove rs the use of these procedures in the study of the mechanisms regulati ng protein glycosylation of several serum proteins from humans and ani mals. Applications of these techniques in fields of biochemical and cl inical interest, such as inflammation, cancer, pregnancy and foetal de velopment, are discussed.