ADVANCES IN AGAROSE-GEL ELECTROPHORESIS OF SERUM-LIPOPROTEINS

Agarose gel electrophoresis has been extensively employed by researche rs to gain a greater understanding of lipoprotein biology and its rela tionship to cardiovascular disease. Advances in this technique have be en made in the visualization and quantitation of separated lipoprotein s, in the use of agarose gel electrophoresis for detection and quantit ation of apolipoproteins of the separated lipoproteins, and in the det ection of lipoprotein heterogeneity. Agarose gel electrophoresis has b een employed for two-dimensional electrophoretic analysis of lipoprote ins as well as in several different methods which probe the immunologi cal properties of lipoproteins. Agarose gel electrophoresis has thus b ecome an important tool in the study of serum lipoproteins in both cli nical and basic science laboratories.