SYNTHESIS OF FLUORESCENT DERIVATIVES OF 3'-O-(6-AMINOHEXANOYL)-PYRIMIDINE NUCLEOSIDES 5'-TRIPHOSPHATES THAT ACT AS DNA-POLYMERASE SUBSTRATES REVERSIBLY TAGGED AT C-3'

Treatment, of 5'-O-dimethoxytritylthymidine 1 and of oxycarbonyl-2'-de oxy-5'-O-dimethoxytrityl-cytidine 12 with a mixture of 6-(benzyloxycar bonylamino)hexanoic acid, dicyclohexylcarbodiimide and 4-(dimethylamin o)pyridine yielded 3'-O-acylated derivatives 2 and 13. These were furt her converted into 3'-O-(6-aminohexanoyl)thymidine 7 and 3'-O-(6-amino hexanoyl)-2'-deoxycytidine 16 monophosphates. The latter compounds wer e labelled at the aliphatic amino group with fluorescent probes and tr ansformed into their triphosphates 9 and 18.