ISOLATION OF DECOMPOSITION PRODUCTS OF TYLOSIN USING LIQUID-CHROMATOGRAPHY

Decomposition products of tylosin A were isolated using open column ch romatography and preparative liquid chromatography. Two decomposition products, formed in slightly alkaline medium, were identified as epime rs of tylosin A aldol, one of which has been described previously. Ano ther decomposition product was formed on exposure of a tylosin A solut ion to light. Isomerization of the double bond between C-12-C-13 takes place, resulting in the formation of the hitherto unreported isotylos in A.