INDIRECT DETECTION IN CAPILLARY ELECTROPHORESIS COMPARISON BETWEEN INDIRECT UV AND INDIRECT LASER-INDUCED FLUORESCENCE DETECTION FOR THE DETERMINATION OF ISOPRENYL PYROPHOSPHATES

Three different detection modes were compared for the determination of isoprenyl pyrophosphates by capillary electrophoresis, viz., direct U V, indirect UV and indirect laser-induced fluorescence (ILIF). The com position of the background electrolyte was varied accordingly. On the basis of separation efficiency (N = 600 000) and limit of detection (c a. 0.5 mu M), the ILIF method gave the best results. Separation was co mplete in less than 6 min.