SYSTEMATIC-APPROACH TO TREATMENT OF ENANTIOMERIC SEPARATIONS IN CAPILLARY ELECTROPHORESIS AND LIQUID-CHROMATOGRAPHY .2. A STUDY OF THE ENANTIOMERIC SEPARATION OF FLUOXETINE AND NORFLUOXETINE

A systematic approach to enantiomeric separations in capillary electro phoresis (CE) and liquid chromatography (LC) with chiral mobile phase additives (MPA) or a chiral stationary phase (CSP) is used in the stud y of fluoxetine and norfluoxetine with cyclodextrins as chiral selecto rs. Binding constants and selectivities are determined under the same experimental conditions (mobile phase, buffer composition). Good agree ment is found between results from the three techniques. The role of t he buffer salt is investigated by comparison of binding constants obta ined with triethylammonium and sodium acetate buffers. Investigation o f the effects of derivatisation of the selector in CE and LC with MPA demonstrates the appropriate choice of cyclodextrin type for use in LC . By studying the influence of organic modifier content on separation parameters, CE can predict a useful solvent working range for a CSP.