DEXTRAN MODIFIED SELF-ASSEMBLED MONOLAYER SURFACES FOR USE IN BIOINTERACTION ANALYSIS WITH SURFACE-PLASMON RESONANCE

The development of a surface modification method based on self-assembl ed monolayers on gold surfaces has proved to be a successful basis for the functionalization of sensor surfaces intended for use in biospeci fic interaction analysis. The dextran coated alkanethiol layer shows h igh chemical stability towards various conditions employed for differe nt analytical sequences. Optimized synthesis sequences yield highly re producible sensor surfaces in terms of surface coverage and biomolecul e binding capacities. Immobilization chemistries adapted for coupling various biomolecules and other ligands were developed for the dextran coated surface and used in bioanalysis techniques based on surface pla smon resonance. This mass sensitive, real-time analysis technique with label-free detection has developed as a universal tool for analysis o f various qualitative and quantitative biospecific interactions, inclu ding affinity and kinetic rate constant determinations.